Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution. Hec ras for mac.
- What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique
- Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Instructions
- Advantages Of Serial Dilution-agar Plate Procedure
Principle of Spread Plate Technique
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The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
Seal and mix the contents gently. Repeat the process with the next tube and plate: Flame and loosen the lids of tubes 1 and 2. Gilles peterson brazilika zip. Transfer 1 ml of liquid from tube 1 to plate -1, and also into tube 2. Serial dilution method for bacterial. Diluted bacteria samples then spread onto the agar plate by L-shaped. The advantages and disadvantages of serial. Advantages And Disadvantages Of Serial Dilution What are the advantages and disadvantages of a serial port? The speed of serial ports are slow. But we can send data on long distances as serial cable have low cost. The Spread Plate Technique, in conjunction with serial dilutions, is a valuable research tool. To demonstrate the cultural characteristics of the bacteria (e.g. Color, texture, size, elevation, etc.). Start studying 13) Serial dilution agar plate. Here's a paragraph about the molds that may help to infer the reason of the advantages during observation of mold colonies: 'Because the structural components of molds are very delicate, even simple handling with an inoculating loop may result in mechanical disruption of their components.The followi ng culture technique es used to to avoid this. The procedure includes a dilution technique which requires spreading a loopful of culture over the agar plate surface. This is to make sure that the individual cells fall apart on the agar medium surface so as separation of the different species takes place.
Procedure of Spread Plate Technique
- Make a dilution series from a sample.
- Pipette out 0.1 ml from the appropriate desired dilution series onto the center of the surface of an agar plate.
- Dip the L-shaped glass spreader into alcohol.
- Flame the glass spreader (hockey stick) over a Bunsen burner.
- Spread the sample evenly over the surface of agar using the sterile glass spreader, carefully rotating the Petridish underneath at the same time.
- Incubate the plate at 37째C for 24 hours.
- Calculate the CFU value of the sample. Once you count the colonies, multiply by the appropriate dilution factor to determine the number of CFU/mL in the original sample.
What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique
Uses of Spread Plate Technique
- It is used for viable plate counts, in which the total number of colony forming units on a single plate is enumerated.
- It is used to calculate the concentration of cells in the tube from which the sample was plated.
- Spread plating is routinely used in enrichment, selection, and screening experiments.
Limitations of Spread Plate Technique
- Strick aerobes are favored while microaerophilic tends to glow slower.
- Crowding of the colonies makes the enumeration difficult.
Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Instructions
References
Advantages Of Serial Dilution-agar Plate Procedure
- M. J. Pelczar
- Mackie and McCartney. Practical Medical Microbiology
- Bailey & Scott’s Diagnostic Microbiology.
- Practical Microbiology. Pradeep Kumar Sharma.